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First, Giddings showed that increased pressure could induce significant changes in molecular volume and the ability of molecules to crowd together to reduce molecular volume upon adsorption. Later on, Guiochon and McCalley reported interesting results observed in isocratic mode and mostly for small-molecule separations. However, the change in retention and selectivity caused by pressure was relatively minor when eluting small solutes (in the pressure range up to 1000 pas).

We are also involved in the development of new column designs and stationary phases which we believe will change the current here practice of protein chromatography.

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Therefore incorrect (hamiş accurate) conclusions have been drawn in several published studies. Our purpose was to dissociate these two contrasting effects and determine their individual contributions to the retention of solutes of various sizes. To realize that, we suggested two sets of experiments: one performed at constant inlet pressure and at varied flow rates, and the other at varied inlet pressure.

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